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. 2017 Mar 15;7:44607. doi: 10.1038/srep44607

Figure 6. STK16 depletion or kinase activity inhibition increases G2/M cell percentage.

Figure 6

(a,b) STK16 depletion increased cells that are positively stained with cyclin B1 and phospho-H3. HeLa cells, as well as HeLa cells stably expressing STK16-GFP-FLAG-WT or MUT (RNAi resistant mutant) were transfected with control or STK16 siRNAs for 72 hours, then they were fixed and stained for anti-GFP (green) and anti-Cyclin B1 (red) antibodies in (a) or with phospho-H3 in (b). (c) Quantification of percentage of G2 phase cells in (a). (d) Quantification of mitotic index after STK16 knockdown in HeLa, HeLa-STK16-GFP-FLAG-WT, and HeLa-STK16-GFP-FLAG-MUT cells. (e) Inhibition of STK16 kinase activity increases cells in G2 phase. HeLa cells were treated with 10 μM STK16-IN-1 for 6 hours, then fixed for immunofluorescence using anti-Cyclin B1 antibody and DAPI. Quantification of percentage of G2 stage cells was shown. The data were analyzed using a student’s t- test (*p < 0.05, **p < 0.01). Data show mean ± SEM, n = 3. Scale bar, 10 μm.