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. 2017 Mar 16;7:44582. doi: 10.1038/srep44582

Figure 2. In vitro primer extension reactions with/without the wild type and mutant PfuPCNAs.

Figure 2

The gel images displayed here are representative of several trials (n = 4). The bar charts at the bottom of the corresponding gel images show the average ratio of the quantities of the elongated primers to that of the unreacted primers and the non-PCNA-assisted-elongation band, as judged from the lane with PfuPolB alone, in which the ratio of the wild type was normalized to 1.0. The borders between the elongated and the unreacted (including non-PCNA assisted elongation) substrates are depicted as dotted lines. The ratios of the DNA substrates to the proteins (PfuPolB and PfuPCNA) in each panel are (A) 1:4, 1:8, and 1:16 (left to right), using the Site A mutants, and (B) 1:4, 1:8, and 1:16 (left to right), using the non-Site A mutants.