Fig. 2.

slo gene expression and activity levels are inversely related. slo transcript abundance relative to cyclophilin 1 as measured by real-time RT-PCR from mutant flies and flies exposed to drugs that induce hyperactivity (gray bars) or sedation (black bars). (A) Relative slo expression in the hyperexcitable mutants eag¹Sh^120b, sei^ts1, Hk¹, and Dppara and in animals treated with toluene (TOL), trichloroethylene (TCE), 0.3% benzyl alcohol (BA#; flies were removed from the benzyl alcohol before entering anesthesia), and 37°C heat shock (Heat). (B) Relative slo expression levels 6 h after sedation with chloroform (CHCl3), CO₂, or 0°C cold anesthesia (Cold). (C) Relative slo expression levels 6 h after tetrodotoxin (TTX) injection. All values are significantly different from control (P < 0.01).