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. 2017 Mar 15;7:44451. doi: 10.1038/srep44451

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Copyright © 2017, The Author(s)

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(a) Immunolocalization of MASP SP and EVs in permeabilized trypomastigotes forms (A. epimastigotes,B.trypomastigotes, C.amastigotes, D. A co-incubation with mice and rat pre-inmune sera as negative controls in free permeabilized E forms) Size bar = 5 μm; N: nucleus; K:kinetoplast. (b). Immunogold labeling with 10 nm of gold particles of MASP SPs in purified EVs. Blue arrows: Non- labeled EVs. Black arrows: labeled EVs. (c) Western blot of ectosomes, exosomes and trypomastigote forms (line 1, 2 and 3 respectively) and their associated densitograms (red, blue and black lines respectively) using anti-EVs antibodies. (d) Quantification and comparison of labelled EVs using Chlatrin and MASP SP antibodies. Bar graphs representative of at least 100 EVs. Size bar = 200 nm.