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. 2017 Mar 15;7:44451. doi: 10.1038/srep44451

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(a) IgG response against the MASP SP and soluble whole protein extract from T. cruzi (TPTC) of a pool of sera from argentinean Chagas disease patients classified as Asymtomatics, Cardiopathy, Digestive and non-infected control group from Bolivia and Argentina. Values with p-valor < 0.001(***) were considered as significantly different. (b) Graphical representation of the antibody-depletion of ICs using a treatment at low pH (4.5). (c) Protein concentrations of free- IgGs EVs (ug/ml) from each serum group after depletion of ICs. The horizontal bar represents mean values and the vertical bar represents SD values. Values with p-valor < 0.0001(****) were considered highly significant. (d) IgG response of each sera group before and after treatment at low pH (with and without ICs) against the MASP SP peptide. Turkey- Kramer test mutilple comparations was used. Values of p < 0.001(***) were considered as significantly different. Cut-off values were obtained calculating the mean of negative controls (sera of non-infected individuals) ± 3 (SEM).