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. 2017 Mar 15;7:44451. doi: 10.1038/srep44451

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(a) Schematic representation of the methodology followed in the immunocapture assays. (b) Detection of MASP SPs (capture antibody) in EVs from Chagas disease clinical group (target) using anti-EVs Trypo immunoglobulins (detection antibody) (c). Detection of free-IgGs EVs from a pool of each Chagas clinical sera group (target), using anti-SP MAPS as capture antibody and a dilution (1/100) of sera without ICs from Chagas disease clinical group as detection antibody. (d) T.cruzi-specificity of EVs in free-IgGs EVs using anti-TPTC immunoglobulin (T. cruzi positive control) and anti-CD9 antibodies (human positive control) as detection antibodies. (e) Detection of parasite EVs (target) in ICs by immunocapture assays using anti-MASP SP antibodies (captured antibody) and a dilution of a pool of chagasic patients’ sera (1/100) (detection antibody). Turkey- Kramer test multiple comparisons was used. Values of p < 0.001 (***) were considered as significantly different. Cut-off values were obtained calculating the mean of negative controls (sera of non-infected individuals) ±3 (SEM).