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. 2017 Mar 15;7:44451. doi: 10.1038/srep44451

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Control for the presence of immunoglobulin in ICs in human sera from chronic chagasic patients and for the absence of Peroxidase enzyme in EVs from ICs (a). Schematic representations of the methodology followed in the controls assays. (b) Determination of human immunoglobulins in ICs in sera from chagasic patients using HRP-conjugated IgG anti-human (Sigma) as detection antibodies (c). Determination of false positives in immunocapture assays by detection of Peroxidase enzyme in free-ICs EVs after low pH treatment. Anti-MASP SP was used as a capture antibody and revealed with the substrate solution for the HRP as detection.