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. 2017 Mar 15;7:44497. doi: 10.1038/srep44497

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Copyright © 2017, The Author(s)

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(A) qPCR analysis of most significantly upregulated ABC transporters. Expression of individual murine ABC transporters was measured using the TaqMan^® Array Mouse ABC Transporters from Life Technologies. Gapdh was used for normalization. Each clone was analyzed in biological duplicate. Stars denote the significance of difference from 4T1 cells; one star, p < 0.05, two stars, p < 0.01. For actual data see Supplementary Table S4. (B) Western blot analysis of the most upregulated ABC transporters. The membrane carrying blotted proteins from cellular extracts of parental and PDT-resistant clones in biological duplicates was sequentially probed with ABCA1, ABCB1 antibodies and eventually with actin antibody to demonstrate equal protein loading.