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. 2017 Mar 15;7:44497. doi: 10.1038/srep44497

Figure 8. siRNA knockdown of ABCB1 leads to accumulation of KP1 and KP6 and restoration of sensitivity to PDT in MCF-7/PacR cells.

Figure 8

MCF-7/PacR cells were transfected with siRNA targeting ABCB1 (+siABCB1) or with non-targeting negative control siRNA (+siNC) and challenged after 72 h with KP1, KP6 and temoporfin (T). (A) The fluorescence following PS treatment was monitored under a fluorescence microscope; pictures were taken under identical camera settings. The phase-contrast images are shown on the left side and PS fluorescence images of the same field on the right side of each column. Scale bar represents 10 μm. siRNA targeting of ABCB1 resulted in a significant increase of PS fluorescence (B), which correlated with an increase of relative PDT sensitivity defined as fold change of LD50 of +siNC-transfected cells/LD50 of +siABCB1-transfected cells (C). Suppression of the ABCB1 protein was confirmed by Western blot (D).