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. 2004 Nov 29;101(49):17228–17233. doi: 10.1073/pnas.0405843101

Fig. 3.

Fig. 3.

Effects of cellular energy on formation of VirB10 complexes. WT or mutant strains were treated with CCCP, arsenate (Ars), or the corresponding EtOH or H2O controls, then detergent-solubilized as described in Experimental Procedures. (A) Material precipitated with anti-VirB10 antibodies from extracts of energized (EtOH- or H2O-treated) or energy-depleted (Ars- or CCCP-treated) WT cells, or the ΔvirB9 (ΔB9) or ΔvirB10 (ΔB10) control strains. (B) Material precipitated with anti-VirB10 antibodies from extracts of energized (H2O) or energy-depleted (Ars) WT cells in the absence (–) or presence of reducing agent (DTT). (C) Material precipitated with anti-VirD4 antibodies from extracts of energized and energy-depleted WT cells or the ΔvirB10 or virD4 control strains. Immunoblots were developed with antibodies to the T4SS subunits listed at the right. Crossreactive material was immunoreactive heavy (filled circle) and light (open circle) chain IgG. T, total cell extract; S, material in supernatant after the immunoprecipitation; IP, immunoprecipitated material.