Fig. 3.

Analyzing transcriptional regulation on yeast promoters using the inducible system. a Screening the effect of different gRNAs for pOLE1-GFP expression relative to a strain without any gRNA expressed. b Screening the effect of different gRNAs for pDGA1-GFP expression relative to a strain without any gRNA expressed. Engineered strains were cultivated with 250 ng/mL of aTc. Maximum values for GFP/OD yield were extracted. c Mean fluorescence intensities (MFI; GFP/OD) from plasmid-based pOLE1-GFP over time targeted with pERA-112 (Sc-170: OLE1 TSS-381 gRNA coupled to dCas9-VPR; dark grey) and pERA-117 (Sc-171: OLE1 TSS-381 gRNA coupled to dCas9-Mxi1; light grey). All results were obtained from three biological replicates monitored with a BioLector over 48 h. MFI values are shown as mean ± S.D. from three (n = 3) biological replicate experiments. For a and b, asterisks indicate significant regulation relative to controls (*p < 0.05)