Fig. 4.

Hierarchical cluster analysis with average-linkage of CHO cells expression levels. a Dendrogram representing the hierarchical clustering of the gene expression levels of the components from the subsystems of ERAD, protein folding, and translocation and the proteasome protein complex. Vertical colour bar: Purple, Proteasome; Blue, ER associated degradation (ERAD); Green, protein folding, and translocation. b Gene expression levels across all samples for components with the functional annotation proteasome clustering in mouse. c Gene expression levels across all samples for components with the functional annotation protein folding in mouse. Grey shadow indicates the position of the components in the hierarchical clustering of the CHO genes. d Gene expression levels across all samples for components with the functional annotation ERAD that clustered in mouse. Horizontal bar, identifier of samples. Top line: protein expressed; no recombinant proteins (grey), IgG (green), and FVIII high levels (dark purple), FVIII medium levels (purple), FVIII low levels (light purple). Middle line: cultivation phase; exponential growth (light blue), stationary phase (Dark blue). Bottom line: NaBu treatment (red)