Figure 3.

Generation of PyMT cell lines from Rag1^−/− and WT hosts. (A) PyMT mice in the Rag1^−/− or WT immune background developed mammary tumors over time. Tumors from each animal were harvested into a single-cell suspension and a cell line was generated. (B) Each cell line was transplanted into five Rag2^−/− x γc^−/− and five WT mice. (C) The kinetic of each cell line in immune deficient Rag2^−/− x γc^−/− vs. immune competent WT mice was compared by determining the difference in time to reach 10×10 mm in size in Rag2^−/− x γc^−/− vs. WT mice. When this value (*) was inferior to 25 d, the cell lines were identified as immune-mediated slow growers. Cell lines for which this value was superior to 25 d, the cell lines were identified as fast growers.