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. 2017 Feb 8;6(2):e1273311. doi: 10.1080/2162402X.2016.1273311

Figure 2.

Figure 2.

Functional and phenotypic characteristics of AdE1-LMPpoly-expanded T-cells (A) The phenotypic characteristics of AdE1-LMPpoly-stimulated T-cells were assessed by measuring the surface expression of CD14 (monocytes), CD19 (B cells), CD16 (NK cells), CD3 (T-cells), CD4 (CD4 T-cells) and CD8 (CD8 T cells). (B) AdE1-LMPpoly-stimulated T-cells were assessed for the intracellular production of IFN-γ following recall with pools of CD8+ T-cell epitopes derived from LMP1&2 or EBNA1. The data represent the frequencies of LMP1&2-specific or EBNA1-specific IFN-γ-producing T-cells in all patients. (C) The data represent the number of patients generating either LMP1&2-specific and/or EBNA1-specific T-cells. (D) The memory characteristics of MHC-multimer+ T-cells were determined by measuring the surface expression of CCR7, CD45RA, CD27, CD28 and CD57. T-cell phenotype was determined as follows: Naïve (TN) CD45RA+CCR7+; Central Memory (TCM) CD45RA−CD27+/−CD28+CD57−; Effector Memory (TEM) CD45RA−CD27−/+CD28−CD57+/−; Effector Memory RA (TEMRA) CD45RA+CD27−/+CD28−/+CD57+.