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. 2016 Dec 27;6(2):e1267094. doi: 10.1080/2162402X.2016.1267094

Figure 6.

Figure 6.

SAP is critical for anti-B cell lymphoma immunity mediated by naive CD8+ Tcells. (A) CFSE-labeled WT (Thy1.1+) and Sh2d1a−/− (SAP−/−, Thy1.2+) OT-I CD8+ T cells were co-transferred at a 1:1 ratio into congenic (CD45.1+) recipients (n = 3) and infected with recombinant L. monocytogenes expressing OVA (+ LM-OVA) one day later. CFSE histograms and distributions of donor WT vs. SAP−/− CD8+ T cells recovered from spleens of LM-OVA infected mice (black histograms) are shown. Gray histograms represent CFSE profiles for donor WT and SAP−/− CD8+ T cells recovered from spleens of naive (uninfected) mice. (B, C) CFSE-labeled WT or SAP−/− OT-I CD8+ T cells (Thy1.1+ CD45.2+) were adoptively transferred into CD45.1+ hosts (n = 6 per group) one day before infusion with B-OVA cells (CD45.2+). (B) Representative donor WT and SAP−/− OT-I CD8+ T cell proliferation and absolute cell number recovery (right) within host spleens at day 9 post-challenge are shown. (C) Splenocytes were left untreated (unstimulated) or re-stimulated (+ PMA/Iono) for detection of surface CD107a and intracellular IFNγ to assess donor CD8+ T cell effector functions. The proportions of donor WT and SAP−/− CD8+ T cells that co-express CD107a and IFNγ upon re-stimulation are presented (bar graph, right). Error bars represent the SD and statistical significance was calculated using unpaired, two-tailed Student's t tests. Triple asterisks (***) indicate p values of less than 0.001. The data in panels B and C are representative of three independent experiments.