Figure 1.

T2 stabilization assay with NQM peptides and analogs. The binding of NLM peptide to HLA-A2 molecule was stronger than NQM and NYM peptides. Native NQM, heteroclitic NLM or NYM peptides were pulsed onto T2 cells at the indicated concentrations as described in the Materials and methods and shown in the inset. The stabilization of the HLA-A2 molecule by the peptides was measured by the expression of HLA-A2 molecule using an anti-HLA-A2 mAb, clone BB7, conjugated to FITC, and recorded as fluorescence on the x-axis.