Figure 5.

Peptide-specific T cell responses and cytotoxicity in HLA- A*24:02⁺ donors. CD3 T cells from a healthy HLA-A*24:02 homozygous donor were stimulated with NQM, NLM or NYM peptides for three rounds. The peptide-specific T cell response was measured by the IFN-gamma secretion upon challenging with individual peptide (A). Each data point represents the mean +/− SD from triplicate cultures. CD3 T cells from two different donors were stimulated with either NQM or NYM peptide for four to five rounds and the cytotoxicity was measured by ⁵¹Cr-release assay against WT1⁺ HLA- A*24:02⁺ mesothelioma cell line (B) leukemia cell line BA25 (C) and the A*24:02 negative line HL60 (D). Each data point represents the mean from triplicate micro-well cultures. Data are representative of 12 experiments from five HLA-A*24:02⁺ donors.