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. 2016 Dec 7;6(2):e1265717. doi: 10.1080/2162402X.2016.1265717

Figure 2.

Figure 2.

Functionality of Ad3-hTERT-CMV-hCD40L in vitro. (A) Schematic presentation of Ad3-hTERT-CMV-hCD40L. (B) Functionality of virally produced hCD40L was confirmed by studying NF-κB/AP-1 activation in Ramos-Blue cells. The assay was repeated three times (in triplicates each time). Oncolytic potency of Ad3-hTERT-CMV-hCD40L in (C) A549 (CD40−) and (D) EJ (CD40+) cells. MTS assay was used to analyze infected cell lines. Cell viability was assessed relative to uninfected mock cells. (E, F) Capability of virally produced hCD40L to induce dendritic cell maturation. The assay was done in triplicate. Lipopolysaccharide; LPS, recombinant hCD40L protein; rhCD40L, supernatant collected from A549 cells infected with Ad3-hTERT-E1A and with Ad3-hTERT-CMV-hCD40L; Ad3, hCD40L, respectively.