Skip to main content
. 2017 Mar 16;7:44265. doi: 10.1038/srep44265

Figure 3. VSOR Cl− channel inhibitors restore cardiomyocyte autophagy impaired by high glucose.

Figure 3

(A–D) Cardiomyocytes were treated with high glucose (33 mM) for indicated times (24, 48, 72 h) or with indicated concentrations of glucose for 72 h. Western blot analysis and quantitative assays were performed. NG, normal glucose. *P < 0.05 v.s. ctrl (NG, 24 h) and #P < 0.05 v.s. NG (72 h) in (A) and (B). *P < 0.05 v.s. NG (5.5 mM) and #P < 0.05 v.s. HG (22 mM) in. (C) *P < 0.05 v.s. NG (5.5 mM) and #P < 0.05 v.s. HG (22 mM) in (D), n = 5. (E) Immunofluorescent staining for cardiomyocytes exposed in normal (Ctrl, 5.5 mM) and high (HG, 33 mM) glucose for 72 h. (F) Western blot analysis of cardiomyocytes treated with high glucose (33 mM), DIDS (100 μM) or DCPIB (5 μM) for 72 h.