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Where indicated, cardiomyocytes were exposed to high glucose (HG, 33 mM) and/or treated with DIDS (100 μM) or DCPIB (5 μM) in the presence of vehicle or 3-MA (2 mM). Cells were then subjected to Western blot analysis (A,D), Annexin V/PI staining and FCM analysis (B, n = 4), and MTT assays (C, n = 5). #P < 0.05 v.s. ctrl (NG); *P < 0.05 versus HG + DIDS group, **P < 0.05 v.s. HG + DCPIB group.
