Fig. 1.

Generation of met mutant mice. (A) Targeting strategy used to insert met cDNA into the met locus. The met expression cassette contains exons 14–20 and poly(A) sequences. Homologous recombination (dashed lines) resulted in the targeted allele. The following mutations were inserted during recombination: M1248T (ATG → ACG), Y1228C (TAC → TGC), D1226N (GAC → AAC), and a double mutation, M1248T/L1193V (ATG → ACG/CTT → GTT). WT met mutant mice also were created. (B) Western blot analysis of Met expression in livers from adult mice. M114 cells (NIH 3T3 cells stably transfected with murine met) were used as a positive control.