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NTHi clinical strain biofilms are highly resistant to amoxicillin treatment. Static biofilms were treated with a range of concentrations of amoxicillin and then viable bacteria quantified by plate count. Results represent the means with standard error of the mean (SEM) of three experiments each done in triplicate. One-way ANOVA measured statistical significance of antibiotic-treated biofilms compared to the vehicle control (*P < 0.05; **P < 0.01; ***P < 0.001).
