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. 2017 Mar 14;5:e3084. doi: 10.7717/peerj.3084

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© 2017 Zhu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) Detection of DAPK promoter methylation in three pairs of random breast cancer patient samples. The methylation-specific PCR products were resolved in 2% agarose gels. M, methylated DAPK; U, unmethylated DAPK; Blank, negative control (without DNA); T, the breast cancer tissue; N, the corresponding adjacent normal tissue. (B) The PCR product sequences inserted into synthetic plasmid. UM, sequence for unmethylated DAPK gene promoter; M, sequence for methylated DAPK genepromoter. (C) Validation of the engineered construct as PCR template. B, blank; V, vector. (D) Quantitative detection of DAPK promoter methylation in breast cancer samples using real-time PCR and. T, breast cancer tumor tissue; N, the corresponding adjacent normal tissue; S1–S15, patient codes.