Table 1.

Summary of primer sequences and product size used in the PCR procedure.

Gene name	Sequences (5′–3′)	Product size (bp)
DAPK methylated	F: GGATAGTCGGATCGAGTTAACGTC	98
	R: CCC TCC CAA ACG CCG A
DAPK unmethylated	F: GGAGGATAGTTGGATTGAGTTAATGTT	106
	R: CAAATCCCTCCCAAACACCAA
DAPK RT PCR	F: GATAGAAATGTCCCCAAACCTCG	189
	R: TCTTCTTTGGATCCTTGACCAGAA
GAPDH RT PCR	F: CGGAGTCAACGGATTTGGTCGTAT	304
	R: AGCCTTCTCCATGGTGGTGAAGAC

Note:

RT PCR, real-time PCR; F, forward primer; R, reverse primer.