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. Author manuscript; available in PMC: 2017 Dec 17.
Published in final edited form as: Neuroscience. 2016 Sep 28;339:64–71. doi: 10.1016/j.neuroscience.2016.09.031

Fig. 3.

Fig. 3

Exposure to intermittent alcohol vapor during adolescence significantly decreased Avp mRNA levels in the PVN of adult male rats (PND 90) compared to controls following an alcohol challenge. Male rats exposed to adolescent intermittent ethanol (AIE) vapor or air (CON) were perfused 2 h after administration of alcohol challenge (4.5 g/kg, ig), and in situ hybridization was performed to quantify Crf and Avp mRNA levels in the PVN. (A) Quantification of PVN Crf mRNA levels by densitometric analysis of autoradiographic signals (arbitrary units, left) and representative dark-field photomicrographs of PVN Crf mRNA levels in adult male rats on PND 90 (100x magnification, right). (B) Quantification of PVN Avp mRNA levels by densitometric analysis of autoradiographic signals (arbitrary units, left) and representative dark-field photomicrographs of PVN Avp mRNA levels in male rats on PND 90 (100x magnification, right). Data in histograms are expressed as mean ± SEM optical density, expressed as percent control relative to CON/EtOH (ethanol) average for the given experiment from 4–6 rats per treatment. *P < 0.05.