Figure 3.

Goa1 mutants of C. albicans have altered cell wall architecture. C. albicans strains: a,f = WT; b,g = GOA31; c,h = GOA32. Differences are noted in the extent and length of the cell surface fibrils with GOA31 compared to the WT cells (“I,” Fig. 3a) and GOA32. Also, the inner cell wall (“II,” Fig. 3a) is more electron dense than that in GOA31, as well as the cell wall layer closest to plasma membrane (white arrow, Fig. 3f). Both are reduced only in GOA31. The average length of cell wall fibrils (d) and inner cell wall (e) are also determined. The fibrils are significantly shorter in GOA31 but the cell wall thickness is similar for all strains. In (d) each data point refers to an collection of 10 measurements of 10 cells for each strain using GIMP2.8 software. Vesicles (circle) in WT (Fig. 3a) are significantly reduced in GOA31. The black arrows may indicate sloughed cell wall material in Figure 3g.³¹ GOA31 = the goa1Δ; GOA32 = the GOA1 reconstituted strain in the GOA31 background.