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. Author manuscript; available in PMC: 2017 Mar 16.
Published in final edited form as: Microfluid Nanofluidics. 2015 Jun 16;19(4):987–993. doi: 10.1007/s10404-015-1608-y

Figure 2.

Figure 2

(a) The average cell diameter for K562 and HL60 cells are 15.5 ± 1.7 μm (n = 114) and 12.4 ± 1.2 μm (n = 36). (b) AFM measurements of cells’ Young’s modulus show that HL60 (E = 0.86 ± 0.22 kPa, n = 24) is stiffer than K562 (E = 0.34 ± 0.21 kPa, n = 114). c) The average values of Young’s modulus and cell diameter are used to calculate cell deformation energy. K562 and HL60 have similar deformation energy (69.8 ± 26.1 kPa·μm3, and 67.6 ± 14.5 kPa·μm3 respectively) when compressed with 9 μm channel gap height. d) AFM measurements of cell relaxation show HL60 (1.45 ± 0.65 s−1, n = 30) is slower in size recovery than K562 (4.42 ± 3.15 s−1, n = 52). t-test was used to analyze statistical significance: **: p < 0.001, ***: p < 0.0001, and ns: no significance. (e) Flow cytometric analysis of cell enrichment using a three-outlet channel. At the outlets, the cell enrichment factor for K562 and HL60 cells are 45.3 and 15.6 respectively. The purity of enriched K562 cells and HL60 cells are 98.7% and 87.9% respectively. The total number of cells initially is 22,000. The number of cells in the K562 outlet, the middle outlet and the HL60 outlet are 11,500, 4600, and 2100 respectively and rounded the nearest hundred.