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. 2017 Jan 25;29(2):310–330. doi: 10.1105/tpc.16.00388

Figure 6.

Figure 6.

PIFs from P. patens Localize to the Nucleus.

(A) Pp-PIF1, Pp-PIF2, Pp-PIF2ΔAPB, Pp-PIF3, Pp-PIF4, and Pp-PIF4ΔAPB localize in the nucleus. Leaves of N. benthamiana were transformed by infiltration with Agrobacterium tumefaciens containing a respective Pro35S:Pp-PIF:YFP construct. One day after transformation, the plants were transferred into darkness for another 1 to 3 d before epifluorescence microscopic analysis (n ≥ 20) of epidermal leaf cells. Bar = 10 µm; in all figure parts, BF = bright field.

(B) Pp-PIF1, Pp-PIF2, Pp-PIF2ΔAPB, Pp-PIF3, Pp-PIF4, and Pp-PIF4ΔAPB colocalize with At-PHYA:NLS in the nucleus. Leaves of N. benthamiana were cotransformed with the respective Pro35S:Pp-PIF:YFP and Pro35S:At-PHYA:NLS:CFP. One day after transformation, the plants were transferred into darkness for another 1 to 3 d and analyzed by epifluorescence /confocal microscopy (n ≥ 20; representative confocal images are shown). Bar = 10 µm.

(C) Pp-PIF1, Pp-PIF2, Pp-PIF2ΔAPB, Pp-PIF3, Pp-PIF4, and Pp-PIF4ΔAPB localize to the nucleus in P. patens. Protonema filaments were transiently transformed with the respective Pro35S:Pp-PIF:YFP using particle bombardment and incubated in darkness for 2 to 4 d before epifluorescence microscopy analysis (n ≥ 13). Arrows indicate nuclei. Bar = 10 µm.