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. 2017 Jan 25;29(2):310–330. doi: 10.1105/tpc.16.00388

Figure 7.

Figure 7.

Effects of PIFs from P. patens on Light Signaling in Arabidopsis.

(A) The response of Arabidopsis seedlings to light is impaired by Pp-PIF overexpression. Col-0 seedlings (control), Col-0 seedlings expressing Pro35S-driven Pp-PIF1:YFP, Pp-PIF2:YFP, or Pp-PIF2ΔAPB:YFP, as well as phyA-211 and phyB-9 seedlings, were grown for 4 d in R (22 µmol m−2 s−1), FR (3 µmol m−2 s−1), or B (8 µmol m−2 s−1) light or in darkness (D). PpPIF2ΔAPB lines 1 and 2 were grown in the same experiment, but on separate plates (for corresponding wild type, phyA-211, and phyB-9, see quantification in [B]). Bar = 5 mm.

(B) Pp-PIF overexpression affects hypocotyl length of Arabidopsis seedlings grown under different light conditions. Col-0, Pro35S-driven Pp-PIF1:YFP, Pp-PIF2:YFP or Pp-PIF2ΔAPB:YFP, phyA-211, and phyB-9 as well as Pro35S-YFP (pPPO30v1HA, empty vector control) expressing seedlings were grown as described in (A) before quantification of hypocotyl length. Data are shown as violin plots; white dots represent the median, and asterisks indicate P values (unpaired, two-tailed Student’s t test) of <0.05 (*), <0.01 (**), and <0.001 (***), respectively. The y axis scale was adjusted for maximal resolution in each plot.

(C) P. patens PIF overexpression results in early flowering in Arabidopsis. Col-0 plants expressing Pro35S-driven Pp-PIF1:YFP, Pp-PIF2:YFP, or Pp-PIF2ΔAPB:YFP, as well as phyA-211 and phyB-9, were grown for 21 d under standard greenhouse conditions. Bar = 1 cm.