Figure 5.

SPT5 Interacts with VIP5.

(A) DNA binding domain (BD) or a BD-SPT5 fusion was tested for its ability to bind to an activation domain (AD) fused to VIP5. The growth of two dilutions (1 × 10⁻¹and 2 × 10⁻²) of the yeast culture on SD medium lacking Trp, Leu, His, and adenine is shown.

(B) SPT5 fused to the N terminus of YFP or the N terminus of YFP alone were tested for their ability to bind to the C terminus of YFP alone or the C terminus of YFP fused to VIP5. Yellow fluorescence (from YFP) and a bright-field image were recorded and the resulting images were merged. DIC, differential interference contrast. Bar = 100 µm.

(C) VIP5 containing the Plus3 domain tested in the yeast two-hybrid analyses is shown.

(D) BD fused with various SPT5 domains shown in (Figure 2A) were tested for their interaction with either AD-VIP5 or AD-Plus3 (C). The growth of two dilutions (1 × 10⁻¹ and 2 × 10⁻²) of the yeast culture on synthetic defined medium lacking Trp, Leu, His, and adenine is shown.