Figure 4.

Characterization of RLA1 Localization, Activity, and Levels, and Induction of RLA1.

(A) RLA1-GFP mainly localized in the nucleus. Bars = 25 μm.

(B) RLA1 has transcription-activation activity in yeast. The RLA1 coding sequence was cloned into the vector pGBKT7. OsBZR1 was used as a positive control. Transformed yeast were serially diluted and placed on SD (synthetic dropout medium) screening plates containing 3-aminotriazole (3AT).

(C) The transcript level of RLA1 in the wild-type plants treated with 1 μM CS (with CS) or control (without CS) for 2 h. Leaves of 2-week-old seedlings were used for the treatment. The transcript level of control was defined as “1.” Data are means ± se (n = 3).

(D) Time course of RLA1-FLAG protein levels in the RLA1ox line after 1 μM CS treatment. The Ponceau S-stained Rubisco large subunit (Rbc L) was used as a loading control.

(E) Quantification analysis for (D). The relative level of RLA1-FLAG at 0 h was defined as “1.” Data are means ± se (n = 3).

(F) RLA1-FLAG protein levels in the RLA1ox line grown on medium containing CS or BRZ for 8 d. Rbc L was used as a loading control.

(G) Quantification analysis for (F). The relative RLA1-FLAG protein level in plants without treatment was defined as “1.” Data are means ± se (n = 3).