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. 2017 Jan 18;29(2):345–359. doi: 10.1105/tpc.16.00650

Figure 2.

Figure 2.

The oscul3a Mutant Displays Enhanced Resistance to Both M. oryzae and Xoo.

(A) and (B) Punch inoculation of oscul3a mutant and ZH11 plants with the compatible M. Oryzae isolate RB22.

(A) Leaves were photographed at 12 dpi.

(B) Lesion area (left) and fungal biomass (right) of the inoculated leaves of oscul3a mutant and ZH11 plants as shown in (A) at 12 dpi. Error bars represent the se; n = 3 (t test between mutant and ZH11 values; *P < 0.05).

(C) and (D) Leaves of oscul3a mutant and ZH11 plants were inoculated with three compatible Xoo isolates.

(C) Leaves at 15 dpi.

(D) Lesion lengths on the leaves at 15 dpi. Error bars represent the se; n = 3 (t test between mutant and ZH11 values; ***P < 0.001).

(E) Total RNA was extracted from the leaves (second from the top) of oscul3a mutant and ZH11 plants at 60 dps. qRT-PCR was used to analyze the expression of OsPR1a, OsPR1b, OsPR10, OsPAL1, OsWRKY45, and OsAOS2. Data were normalized to the expression level of the constitutively expressed OsACTIN gene. Error bars represent the se; n = 3 (t test between mutant and ZH11 values; *P < 0.05, **P < 0.01, and ***P < 0.001).