Figure 4.

NRP1 Directly Interacts with Transcription Factor WER in Vitro and in Vivo.

(A) Pull-down assay. Protein extracts from transgenic plants expressing YFP-NRP1 were incubated with beads coated with GST and GST-WER. Quantity/purity of GST and GST-WER beads were analyzed by SDS-PAGE gel stained by Coomassie blue (CBB) (left panel). Two percent of the input and pull-down fractions were analyzed by immunoblot using polyclonal anti-GFP antibody (indicated by the arrowhead, right panel).

(B) BiFC analysis of interaction between NRP1 and WER in tobacco leaf cells. Bars = 50 μm.

(C) SEC profiles (Superdex 200 10/300 GL) of NRP1 (blue) and WER (red), as well as their mixture at a molar ratio of 1:1 (black).