Figure 8.

NRPs Promote Histone Release and Decrease Nucleosome Density at GL2 Promoter.

(A) Schematic representation of structures of GL2 promoter and first two exons.

(B) and (C) Histone occupancy at promoter regions of GL2. Roots of vertically grown wild type and nrp1-1 nrp2-1 at 12 DAG were fixed with formaldehyde for ChIP analysis using commercial antibodies against H2B (B) and H3 (C). Error bars show sd from three biological replicates. Asterisks indicate statistically significant difference of histone enrichment at GL2, FLC, and ACT2 between the wild type and nrp1-1 nrp2-1 (P < 0.05).

(D) Nucleosome density at the GL2 promoter in nrp1-1 nrp2-1. Nuclei were isolated from roots of the wild type and nrp1-1 nrp2-1 at 12 DAG and were treated with MNase. The digested DNA fragments were purified for quantitative PCR analysis, and the intact genomic DNA without digestion were used as the input. Error bars show sd from three biological replicates. Asterisks indicate statistically significant difference of DNA level between the wild type and nrp1-1 nrp2-1 (P < 0.05).