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TEAR1 and TEAR2 Have E3 Ligase Activity.
E3 Ub ligase activity was tested using MBP-tagged TEAR1 (A) or MBP-TEAR2 (B) in the presence or absence of E1, E2, and Ub. An anti-Ub antibody was used to detect ubiquitinated proteins (top), and an anti-MBP was used to determine the amounts of MBP-TEAR1, MBP-TEAR2, MBP, MBP-mTEAR1, and MBP-mTEAR2 controls (bottom). Molecular mass markers in kilodaltons are indicated on the left.
