Figure 7.

Disruption of TEAR1 Family Proteins Leads to Defective Leaves and Genetic Interaction between TEARs and TCPs.

(A) to (D) Leaf phenotypes of 28-d-old wild type and three independent 35Spro-mTEAR1 transgenic lines. Bars = 1 mm

(E) Close-up views of the leaves from 28-d-old wild-type and two independent 35Spro-mTEAR1 transgenic plants. From left to right, the 5th and 6th leaves from the wild type and 35Spro-mTEAR1 transgenic lines. Bars = 1 mm.

(F) Phenotypes of 32-d-old wild type and two tear1 tear2 mbr1 mbr2 mbrl1 mbrl2 sextuple mutants. Bars = 1 mm.

(G) Close-up views of the 6th and 7th leaves from 32-d-old wild-type and two tear sextuple mutants. Bars = 1 mm.

(H) Phenotypes of 33-d-old wild type, VC330, jaw-5D, and VC330 jaw-5D double mutant. Bar = 1 cm.

(I) Close-up views of the 5th and 6th leaves from 33-d-old wild-type, VC330, jaw-5D, and VC330 jaw-5D double mutant. Bar = 1 mm.

(J) Phenotypes of 25-d-old wild type, VC330, 35Spro-mTCP4, and VC330 35Spro-mTCP4 double mutant. Bar = 1 cm.

(K) Close-up views of the 5th and 6th leaves from 25-d-old wild type, VC330, 35Spro-mTCP4, and VC330 35Spro-mTCP4 double mutant. Bar = 1 mm.

(L) Relative expression levels of the known TCP direct target genes in the leaves of wild type, VC330, and 35Spro-mTCP4. The expression levels of the genes in wild-type plants were set to 1.0. The error bars represent the sd of three biological replicates. Total RNA was extracted from the mixed leaf tissues of six wild-type, VC330, or 35Spro-mTCP4 plants. After reverse transcription, real-time PCR was performed three times to determine the relative expression levels of the TCP direct target genes.