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. 2017 Mar 16;12(3):e0169687. doi: 10.1371/journal.pone.0169687

Fig 2. Microarray analysis of bone marrow macrophages from the cmo mouse model.

Fig 2

(A) Gene expression heatmap from microarray analysis of RNA from bone marrow-derived macrophages of cmo, IL-1R-/- and IL-1R+/- mice (n = 3 for each strain). Normalized expression values were analyzed for differential expression using Partek Genomics Suite software (version 6.12, Partek Inc., St. Louis, MO, USA.). The top 25 differentially expressed genes are shown. Fblim1 (red arrow) was the most differentially expressed (downregulated) gene in the cmo mouse. (B, C and D) Representative fixed decalcified tail bone sectioned and stained with H&E from cmo, IL-1R-/- and IL-1R+/- mice, respectively. The cmo mouse has extensive mixed inflammatory infiltrate with destruction of the vertebral body, the cmo.IL-1R+/- has similar but less severe inflammation and bone destruction, whereas, the cmo.IL-1R-/- mouse has normal bone with no inflammation. Representative tail kinks and foot deformities in these mice were depicted and described previously [10].