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. 2004 Dec 10;101(51):17789–17794. doi: 10.1073/pnas.0406111101

Fig. 4.

Fig. 4.

BLyS fails to induce G1/S transition. (A) Immunoblot of p27, CDK2, and cyclin E in resting p18+/+ and p18-/- B cells cultured for 0 or 48 h as indicated. The level of actin was used for a loading control. (B) FACS analysis of BrdUrd uptake in resting p27+/+ and p27Δ51/Δ51 B cells cultured in the absence or presence of BLyS, 33 nM (1×) or 66 nM (2×) of HIV-1 transactivator of transcription cyclin E (E), or both, for 48 h in the presence of the lipopolysaccharide inhibitor. Similar results were obtained from five independent experiments regardless of the presence of the lipopolysaccharide inhibitor.