Table 3. Number of identified residue pairs added (at 5% FDR) with alternatives to trypsin-only digestion.

Target protein	5% FDR total	Glu-C digestion a	% Glu-C identified of total
Tx781 (420 AA)	305	119	39%
Of this in N-term. 180 AA	77	57	74%
Tx808 (418 AA)	265	45	17%
Tx767 (318 AA)	381	111	29%
Tx812 (204 AA)	201	36	18%

^aAll digestion methods involving use of Glu-C (including trypsin/Glu-C co-digestion (Tx781 and Tx808), and in-solution Glu-C digestion (Tx808, Tx767 and Tx812).