Fig 3. Comparison of hsa-miR196a-2 expression at genotype level between controls and patients.

Total RNA was isolated from colon biopsy samples from healthy subjects and UC patients and reverse transcribed to cDNA using specific primers. Data were derived from quantitative real-time PCR. Normalization of samples was performed with the U6 snRNA. Sample size was 8–12 samples in homozygous CC and heterozygous CT in each category. For homozygous mutant n = 4 for UC. Box plots present median ± 25th and 75th percentiles (solid box) with the lowest and highest percentiles shown by whiskers outside the box. The Ct values were subtracted from 20, so higher values represented higher mRNA expression levels. Significance values are represented as * p ≤ 0.05, *** p ≤ 0.001.