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. 2017 Mar 8;37(10):2524–2538. doi: 10.1523/JNEUROSCI.2661-16.2017

Figure 2.

Figure 2.

AnkG is required for nodal maturation but not initial assembly and protein clustering at the nodes. A–I, Immunostaining of SN fibers from P5, P10, and P30 age-matched Ankfx/− and SLICK-H-CreER;Ankfx/− littermate mice with antibodies against AnkG (blue) in combination with the following proteins: NF186 (A–C), βIV Spec (D–F), or pan-NaV (G–I; red) and Caspr (green). J–L, Quantification of NF186, βIV Spec, or pan-NaV intensity, respectively, in the SN nodal area standardized to the same age control values from P5, P10, and P30 Ankfx/− (black bars), and SLICK-H-CreER;Ankfx/− (red bars) mice (n = 3 mice/genotype; 60–80 nodes/animal; unpaired, two-tailed Student's t test). M–U, SCs from P5, P10, and P30 age-matched Ankfx/− and SLICK-H-CreER;Ankfx/− littermate mice were immunostained with antibodies against AnkG (blue) in combination with either of the following proteins: NF186 (M–O), βIV Spec (P–R), or pan-NaV (S–U; red), and Caspr (green). V, W, X, Intensity of NF186, βIV Spec, or pan-NaV, respectively in the SC nodal area standardized to the same age control values from P5, P10, P30 Ankfx/− (black bars), and SLICK-H-CreER;Ankfx/− (red bars) mice (n = 3 mice/genotype; 60–80 nodes/animal; unpaired, two-tailed Student's t test). Y, Z, Percentages of normal and patchy nodes in SN and SC, respectively, with remaining NF186, pan-NaV, and βIV Spec at P10 and P30 in SLICK-H-CreER;Ankfx/− mice compared with Ankfx/− controls (n = 3 mice/genotype; 60–80 nodes/animal; comparison of normal nodes performed by two-way ANOVA with Bonferroni's post hoc analysis). Arrowheads mark AnkG-negative nodes in the SLICK-H-CreER;Ankfx/− mice. Scale bar, 2 μm. All data are represented as the mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.