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. 2017 Mar 8;37(10):2709–2722. doi: 10.1523/JNEUROSCI.4559-15.2017

Figure 6.

Figure 6.

ARPP-16/19 does not regulate Ser845 phosphorylation of GluA1 or Thr34 phosphorylation of DARPP-32. Striatal slices from ARPP-16/19 cKOs (n = 4) or WT littermate controls (n = 3 or 4) were isolated and basal phosphorylation of the PP2A targets, (a) elongation factor-2 (EF2) at Thr56 or (b) tau at Ser396, were analyzed by immunoblotting. a, Phospho-Thr56 (pEF2) and total EF2 (TotEF2) blots. b, Phospho-Ser396 (pTau) and total tau (TotTau) blots. Bar graphs represent cumulative data (multivariate ANOVA p > 0.05). Data for cKO mouse samples were normalized to controls. Striatal slices from ARPP-16/19 cKOs (n = 4) or WT littermate controls (n = 4) were incubated with 10 μm forskolin for 0, 10, 30, or 60 min. Samples were analyzed by immunoblotting for (c) phospho-Ser845 and total GluA1 and (d) phospho-Thr34 and total DARPP-32 (bottom); quantitation of phosphorylation was calculated for Ser845 or Thr34 normalized to total GluA1 or total DARPP-32, respectively. Graphs represents cumulative data (multivariate ANOVA, p > 0.05). Data for cKO mouse samples were normalized to controls.