Figure 3.

CSF influx is globally impaired after multiple microinfarcts. CSF tracer influx was by ex vivo whole-slice imaging 30 min after intracisternal infusion of fluorescent tracer. A–C, Representative images showing that, 3 d after multiple microinfarcts (B), CSF tracer influx is globally impaired compared with sham-treated controls (A). C, CSF tracer influx is dramatically impaired 30 min after tracer infusion, whereas within 3 h of infusion, CSF tracer has entered into the injured brain (**p < 0.01, t test; n = 4–5 per group). D, At 14 d after injury, no significant differences in CSF tracer intensity are observed between animals subjected to multiple microinfarcts or sham operation. Scale bars in A and B, 500 μm.