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. 2017 Mar 15;37(11):2931–2946. doi: 10.1523/JNEUROSCI.3316-16.2017

Figure 2.

Figure 2.

Myelin gene expression is reduced in mice lacking Fgfr2 but not Fgfr1. A, Transverse sections of cervical spinal cord at P30, analyzed by in situ hybridization for MBP and PLP mRNA from control, Fgfr1 cKO, Fgfr2 cKO, and Fgfr1/Fgfr2 dKO mice show reduced signal intensity for MBP mRNA in Fgfr2 cKO and Fgfr1/Fgfr2 dKO but not Fgfr1 cKO mice compared to controls. PLP mRNA signal also appears to be reduced in Fgfr2 cKO and Fgfr1/Fgfr2 dKO but not Fgfr1 cKO oligodendrocytes compared to controls (inset shows cellular labeling at higher magnification). B, Quantification of mRNA levels by qRT-PCR in P15 spinal cords shows a significant reduction in the expression of MBP, PLP, and CGT mRNA levels in Fgfr2 cKO (R2KO) and Fgfr1/Fgfr2 dKO (dKO) but not Fgfr1 cKO (R1KO) mice compared to controls (cont.). Three to four mice of each group were analyzed. Error bars indicate SEM (N = 3–4). **p < 0.01. WM, White matter; GM, gray matter.