Figure 2.

miR-210 inhibition protects mitochondrial function and downregulates apoptosis in Dcx⁺ cells. miR-210 inhibition attenuated the decrease in mitochondrial cytochrome c oxidase (A) and aconitase activities (B) induced by CM inflammatory exposure. Representative images of apoptotic TUNEL staining (green) in control (C) and miR-210 inhibited (D) NSC cultures without inflammatory injury. Dcx⁺ cells are immunostained in red. CM inflammatory injury significantly increases the level of TUNEL staining (E) and this is attenuated in NSC cultures transfected with miR-210 inhibitor (F). G, Quantification of TUNEL staining. The data show three independent experiments, with at least four samples per condition in each experiment *p < 0.05 compared with no injury control, #p < 0.05 compared with control-transfected cultures after injury; &p < 0.05, &&p < 0.01 compared with miR-210-inhibitor-transfected cultures without injury. Scale bar, 50 μm.