Figure 4.

Early (1–2 d after the start of differentiation) miR-210 inhibition reduces cell density but not the proportion of Dcx⁺ cells. NSC cultures transfected with negative control (A) demonstrate significantly higher cell density compared with miR-210-inhibitor-transfected cultures (B). Quantification of the total cells/field and Dcx⁺ cells/field (C) after 4–5 d of differentiation. The data shown represent three independent experiments, with at least four samples per condition in each experiment. **p < 0.01, ***p < 0.001 compared with control-transfected. Scale bar, 50 μm.