Figure 5.

miR-210 inhibition induces changes in metabolic activity and mitochondrial function. miR-210 inhibition suppresses glucose consumption (A) and lactate production rates (B) when assessed after 3 h. Glucose consumption and lactate production are both indicators of glycolytic activity. C, D, Representative images of mitochondrial TMRE staining in NSC cultures transfected with negative control (C) and miR-210 inhibitor (D). Quantification of changes in TMRE fluorescence as a measure of mitochondrial potential (E) and mitochondrial morphology (fraction of cell mitochondria <3 μm² size) (F). The data show three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 compared with control-transfected condition. Scale bar, 25 μm.