Figure 7.

Mitochondrial inhibition by oligomycin eliminates the miR-210 inhibitor-associated decrease in NSC density. Left panels show nuclear DAPI staining, right panels show CellRox fluorescence. miR-210 inhibition leads to decreased cell densities under normal differentiating conditions (A, B), but does not change NSC densities in oligomycin (2 μm)-treated cultures (C, D). See text for quantification of cell density in oligomycin-treated cultures. Quantification shows oligomycin-induced increase in cellular ROS, but no difference in ROS levels between miR-210 inhibitor and control-transfected cultures (E). The data show three independent experiments, with at least six samples per condition in each experiment. ***p < 0.001 compared with the corresponding conditions without oligomycin treatment. Scale bar, 50 μm.