Figure 8.

Early (days 1–2 of differentiation) transfection with miR-210 mimic decreases Dcx⁺ cell density. A, B, NSC cultures transfected with miR-210 mimic demonstrated comparable total cell densities, but decreased Dcx⁺ cell numbers compared with negative control-transfected cultures on days 4–5 of differentiation (nuclei are labeled with DAPI in blue; Dcx⁺ cells are green). C, Quantification of total and Dcx⁺ cell numbers. The data show three independent experiments with at least three samples per condition in each experiment. #p < 0.05 compared with control. Scale bars, 50 μm.