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Selective expression of Neurod6 in mDA neurons in the VTA. A–D, In situ hybridization for Neurod6 combined with immunohistochemistry for TH showing that Neurod6 expression is restricted to TH+ mDA neurons located in ventral regions of the VTA from E15.5 to adult stage (A–C). In contrast, Neurod6 is not expressed in TH+ mDA neurons in the SNc (D). A′–D′, Higher magnification of boxed regions in corresponding panels A–D. IPN, interpeduncular nucleus. Scale bars: A, 200 μm; A′, 10 μm.
